This medium-ACF is a serum-free medium designed for optimal growth of human embryonic stem cells and induced pluripotent stem cells under feeder-free conditions. It is a sterile, liquid medium containing essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors and trace minerals. The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/ 95% air. This medium-ACF is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes growth of normal human embryonic stem cells and induced pluripotent stem cells in vitro. This medium should be used in conjunction with BD Matrigel™ for feeder-free culture condition.
Storage and Shipping
Store the basal medium and complete medium-ACF at 4 °C. Store 50X at -20 °C. Protect from light. Gel ice.
This medium-ACF consists of 500 ml of This medium basal medium and 10 ml of 50X human pluripotent stem cell growth supplement.
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