Porcine Mammary Epithelial Cells
Cat.No.: CSC-C9248J
Species: Pig
Source: Breast
Cell Type: Epithelial Cell
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Porcine Mammary Epithelial Cells are primary cells isolated from the mammary gland tissue of pigs and are used as an in vitro model to study mammary gland biology, lactation physiology, and epithelial cell function. In vivo, mammary epithelial cells form organized ductal and alveolar structures supported by surrounding stromal tissue. They respond to hormonal regulation, including prolactin, estrogen, and progesterone, which coordinate mammary gland development, lactogenesis, and involution. Porcine mammary tissue shares physiological and anatomical similarities with that of other large mammals, making these cells useful for comparative and translational research in reproductive biology and agricultural science.
In culture, Porcine Mammary Epithelial Cells typically exhibit a cobblestone-like epithelial morphology and grow as adherent monolayers under defined conditions. When provided with appropriate extracellular matrix components and differentiation media, they can express milk protein genes and maintain epithelial-specific markers, supporting functional studies of secretion and barrier properties. These cells are widely applied in research on mammary gland development, mastitis and inflammatory responses, host-pathogen interactions, milk production mechanisms, and nutrient transport. They are also used in studies of epithelial cell signaling, tight junction regulation, and tissue engineering approaches. Although primary cells have limited lifespan and may show donor variability, Porcine Mammary Epithelial Cells provide a relevant system for investigating mammary epithelial biology in vitro.
Taurine Attenuates H2O2-induced Oxidative Stress
Oxidative stress reduces mammary gland health and milk production ability in late-gestation and lactating sows. Dietary supplementation with taurine increases antioxidant capacity and offspring growth performance but mechanisms are unknown. Xu's team examined taurine-mediated cytoprotection against oxidative stress injury and signaling pathways involved in porcine mammary epithelial cells (PMECs).
First, they sought to establish concentrations of H₂O₂ to use for inducing oxidative stress. PMECs were treated with 0-1000 μM H₂O₂ for 12-24 hours. Cell viability decreased and ROS production increased in a concentration-dependent manner with H₂O₂ treatment (Fig. 1a, b, d, e). Treatment with H₂O₂ (500 μM) for 12 hours decreased cell viability by ~40% and decreased T-SOD and increased ROS production compared to control cells (Fig. 1e, f). Therefore, the authors used PMECs treated with 500 μM H₂O₂ for 12 hours to induce oxidative stress injury.
To evaluate cytoprotection mediated by taurine against oxidative stress, PMECs were pretreated with 0-2 mM taurine for 12 hours followed by treatment with 500 μM H₂O₂. Pretreatment with 0-1.2 mM taurine decreased cell death in a concentration-dependent manner (Fig. 2a). Pretreatment with 0.8-1.2 mM significantly decreased ROS production compared to cells treated with H₂O₂ alone (Fig. 2b). The greatest response was seen with 1.2 mM taurine. Furthermore, 0.8 mM significantly increased T-SOD compared to H₂O₂ treatment alone (Fig. 2c). Confirmatory experiments verified that treatment with 1.2 mM taurine significantly attenuated ROS production by H₂O₂ (Fig. 2d).
(1) When thawing serum, please follow the recommended stepwise thawing method (-20°C to 4°C to room temperature). If the temperature is changed too much when thawing serum (e.g. -20°C to 37°C), precipitates will be very easily generated. (2) When thawing serum, please shake it evenly at any time so that the temperature and composition are uniform to reduce the occurrence of precipitation. (3) Do not leave the serum at 37°C for too long. If left at 37°C for too long, the serum will become cloudy and many unstable components of the serum will be damaged, thus affecting the quality of the serum. (4) Heat inactivation of serum is very likely to cause an increase in precipitates, so this step may not be necessary if it is not necessary. (5) If heat inactivation of serum must be done, please observe the 56°C, 30-minute rule and shake well at all times. Too high a temperature, too long a time or uneven shaking can cause an increase in precipitate.
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Average Rating: 5.0 | 1 Scientist has reviewed this product
Excellent value for money
We also received a lot of freebies when we purchased the products. It's really good value for money!
15 Mar 2023
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