Porcine Mammary Epithelial Cells

Specification
Recommended Products

Cat.No.

CSC-C9248J

Description

Porcine Mammary Epithelial Cells from Creative Bioarray are isolated from breast tissue of porcine. Porcine Mammary Epithelial Cells are grown in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray’s Culture Complete Growth Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml and is delivered frozen. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.

Species

Porcine

Source

Breast

Recommended Medium

Complete Epithelial Cell Medium

Cell Type

Epithelial

Disease

Normal

Storage and Shipping

We ship frozen cells on dry ice. Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. Never can primary cells be kept at -20 °C.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.

Q & A
Customer Review

How to avoid sedimentation?

(1) When thawing serum, please follow the recommended stepwise thawing method (-20°C to 4°C to room temperature). If the temperature is changed too much when thawing serum (e.g. -20°C to 37°C), precipitates will be very easily generated. (2) When thawing serum, please shake it evenly at any time so that the temperature and composition are uniform to reduce the occurrence of precipitation. (3) Do not leave the serum at 37°C for too long. If left at 37°C for too long, the serum will become cloudy and many unstable components of the serum will be damaged, thus affecting the quality of the serum. (4) Heat inactivation of serum is very likely to cause an increase in precipitates, so this step may not be necessary if it is not necessary. (5) If heat inactivation of serum must be done, please observe the 56°C, 30-minute rule and shake well at all times. Too high a temperature, too long a time or uneven shaking can cause an increase in precipitate.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Excellent value for money

We also received a lot of freebies when we purchased the products. It's really good value for money!

15 Mar 2023

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