10% horse serum may be used instead of fetal bovine serum in medium; tested and found negative for ectromelia virus (mousepox)Tumorigenecity:Isoenzyme:Histopathology:Subculture:Split ratio:Media change:Reverse transcritase:Production
STR DNA Profile
NCTC 135 medium, 90%; heat inactivated fetal bovine serum (FBS), 10%; adjust pH to circa 7.2 with humidified 5 to 10% CO2 in air
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