C57BL/6 Mouse Primary Alveolar Epithelial Cells
Cat.No.: CSC-C4276X
Species: Mouse
Source: Lung
Cell Type: Epithelial Cell
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Mouse Primary Alveolar Epithelial Cells can be used in assays of cell to cell adhesion and migration. Standard biochemical procedures performed with epithelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.
C57BL/6 Mouse Primary Alveolar Epithelial Cells are primary cells isolated from murine lung alveolar tissue and serve as an in vitro model to study normal pulmonary structure and function. Isolated cells are predominantly composed of alveolar type I (AT I) epithelial cells and alveolar type II (AT II) epithelial cells.
Mouse Primary Alveolar Epithelial Cells closely resemble in vivo alveolar epithelium and maintain structural markers identified with these lineages, including aquaporin-5 and podoplanin in AT I cells and surfactant protein-A (SP-A), SP-B, and SP-C in AT II cells. When cultured under appropriate conditions, Mouse Primary Alveolar Epithelial Cells maintain surfactant production with AT II cells able to undergo transdifferentiation towards an AT I phenotype.
Mouse Primary Alveolar Epithelial Cells have been utilized to assess mechanisms involved in lung development and maturation, pulmonary inflammation and acute lung injury, lung fibrosis, and respiratory toxicology. Additionally, these cells can be used to measure drug transport and assess inhalation toxicity. Mouse Primary Alveolar Epithelial Cells have also been applied to study host-pathogen interactions. Overall, these cells serve as an effective model system with high physiological relevance.
Real-Time Analysis of Cell Growth of Primary Alveolar Epithelial Cells
Graphene-based nanomaterials are highly conductive, mechanically resistant, and elastic, making them suitable for various applications. Bavorova et al. evaluated the toxicological effects of graphene nanoplatelets (GPs) in vitro and in vivo, focusing on cytotoxicity, cell proliferation, and histopathological changes.
The potential anti-proliferative effect on C57BL/6 Mouse Primary Alveolar Epithelial Cells (PAECs) was assessed using real-time cell growth analysis. The cell index (CI), reflecting cell morphology, adhesion, and viability through electrical impedance, indicates cell adhesion strength and number. After 24 hours of exposure to GPs (Graphene Oxides), the CI of PAECs remained stable or increased, suggesting maintained cell monolayer and enhanced cell-cell attachment (Fig. 1). However, a significant CI decrease was observed with high GP concentrations (50 and 100 µg/mL) and prolonged exposure (48 h), dropping to 0.5 within the first 9 hours and remaining constant thereafter (Fig. 2). This indicates that GPs induced oxidative stress, disrupting the cell barrier and causing a discontinuous cell monolayer.
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