C57BL/6 Mouse Bone Marrow Endothelial Progenitor Cells are derived from the tibias and femurs of pathogen-free laboratory adult mice. C57BL/6 Mouse Bone Marrow Endothelial Progenitor Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Creative Bioarray's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 1x10^6 cells per ml and are delivered frozen.
Standard biochemical procedures performed with progenitor cell cultures include cell adhesion, migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request.
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