ActoFactor™ Recombinant Human Transforming growth factor, beta receptor II (70/80kDa)

CSC-CTK0333

TGFBR2 is part of the Ser/Thr protein kinase family and the TGFB receptor subfamily. TGFBR2 is a transmembrane protein that has a protein kinase domain, forms a heterodimeric complex with another receptor protein, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. Mutations in TGFBR2 gene have been associated with Marfan syndrome, Loeys-Deitz Aortic Aneurysm Syndrome, and the development of various types of tumors. TGFBR2 expression is increased in oral squamous cell carcinoma cells. TGFBR2 attenuates the biological activities of TGF-beta in colorectal cancer. TGFBR2 expression is decreased by IL-1beta while inducing Sp3 via NFkappaB. TGFB2 and TGFBR2 are involved in the antiestrogenic activity of tamoxifen metabolites in breast cancer. Human Recombinant TGFBR2 produced in E. coli is a single, non-glycosylated, polypeptide chain encoding 133 amino acids (4-136 aa, the extracellular domain of TGFBR2) with a molecular mass of 15.1 kDa.

Human

Human TGFBR2 expressed in E.coli

15.1 kDa

CAT# CSC-CTK0333-10 (10 μg); CAT# CSC-CTK0333-50 (50 μg)

E.coli

Greater than 97% as determined by SDS-PAGE analysis.

Measured by its ability to inhibit TGF-b1 activity on a mouse T cell line, HT-2.

N/A

Lyophilized from a sterile-filtered solution in buffer containing 150 mM NaCl and 50 mM sodium phosphate buffer (pH 7.5).

Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0,1 - 1,0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rRa Leptin should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

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