ActoFactor™ Recombinant Human Interleukin 9 (E. coli)
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Cat.No.
CSC-CTK0241
Description
Recombinant Human Interleukin-9 (rHu IL-9) is a T-cell derived growth factor produced preferentially by CF4+ helper cells. rHu IL-9 is a pleiotropic cytokine with multiple functions on cells of lymphoid, myeloid, and mast cell lineages. Human IL-9 is a 14 kDa protein containing 127 amino acid residues.
Species
Human
Product Overview
Human IL9 expressed in E.coli
Molecular Mass
14.0 kDa
Size
CAT# CSC-CTK0241-10 (10 μg); CAT# CSC-CTK0241-50 (50 μg)
Expression System
E.coli
Purity
Greater than 98% as determined by SDS-PAGE, FPLC and HPLC analysis.
Activity
The ED50 as determined by the dose-dependent proliferation of human MO7e cells is 0.2 ng/ml, corresponding to a specific activity of 5×10^6 units/mg.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Lyphilized from a sterile-filtered, concentrated (1 mg/ml) protein solution with no additives.
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized rHu IL-9 should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu IL-9 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.
Citation Guidance
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