ActoFactor™ Recombinant Human Chemokine (C-X3-C motif) ligand 1
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Cat.No.
CSC-CTK0558
Description
Human Fractalkine is a member of the newly discovered CX3C family of chemokines which contains both chemokine and mucin domain. Human Fractalkine is an 8.638 kDa protein containing 76 amino acid residues. It has been purified using proprietary chromatographic techniques.
Species
Human
Product Overview
Human CX3CL1 expressed in E.coli
Molecular Mass
8.6 kDa
Size
CAT# CSC-CTK0558-20 (20 μg); CAT# CSC-CTK0558-100 (100 μg)
Expression System
E.coli
Purity
Greater than 97% as determined by SDS-PAGE and RP-HPLC analysis.
Activity
Determined by its ability to chemoattract human T cells using a concentration range of 5.0-10.0 ng/ml.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Lyophilized from a sterile-filtered solution in 20 mM phosphate buffer (pH 7.4) and 50 mM NaCl.
Reconstitution
Please centrifuge product briefly before opening the vial. Reconstitute in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted Fractalkine should be used immediately or stored in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.
Citation Guidance
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