ActoFactor™ Recombinant Rat Epidermal growth factor receptor

CSC-CTK0803

EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-α and VGF (vaccinia virus growth factor). Recombinant rat EGF is a 6.2 kDa globular protein containing 54 amino acid residues including 3 intramolecular disulfide-bonds.

Rat

Rat Egfr expressed in E.coli

6.2 kDa

CAT# CSC-CTK0803-100 (100 μg); CAT# CSC-CTK0803-500 (500 μg)

E.coli

Greater than 98% as determined by SDS-PAGE and HPLC analysis.

The ED50 was determined by a cell proliferation assay using BALB/c 3T3 cells is 1 x 10^7 units/mg.

Less than 1 EU/μg.

Lyophilized from a sterile-filtered solution with no additives.

Please centrifuge product before opening vial. The lyophilized protein should be reconstituted with distilled, sterile water to a concentration of 0.1-1.0 mg/ml. For further dilution, carrier protein (0.1% BSA or HSA) should be added to avoid loss of bioactivity.

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.