Interleukin-7 (IL-7) is a stromal cell-derived growth factor produced in bone marrow, thymus and spleen. IL-7 promotes the proliferation of precursor B-cells, thymocytes, T cell progenitors and mature CD4+ and CD8+ T cells. IL-7 can induce the formation of lymphokine-activated killer (LAK) cells as well as the development of cytotoxic T lymphocytes (CTL). Recently, IL-7 was also shown to induce the V(D)J rearrangement of the T cell receptor b gene in mouse fetal thymocytes. Among myeloid lineage cells, IL-7 can up-regulate the production of pro-inflammatory cytokines and stimulate the tumorocidal activity of monocytes/macrophages. IL-7 binds to a high affinity receptor formed by the ligand binding subunit (IL-7 R) and the g chain of the IL-2 receptor. Mouse IL-7 cDNA encodes a precursor protein of 154 amino residues containing a 25 amino acid residue signal peptide. The methionyl form of recombinant mouse IL-7 contains 130 amino acid residues and has a molecular mass of approximately 15 kDa.
Mouse Il7 expressed in E.coli
A DNA sequence encoding the mature mouse IL-7 protein was expressed in E. coli.
CAT# CSC-CTK0029-5 (5 μg); CAT# CSC-CTK0029-25 (25 μg)
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity was determined by T cell growth factor activity in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes and the ED50 for this effect was typically 0.15 - 0.3 ng/mL.
Endotoxin level less than 0.1 ng per 1 mg of the cytokine as determined by the LAL method.
Lyophilized from a sterile solution in PBS containing 50 mg of bovine serum albumin per 1 mg of cytokine.
It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 1 mg/mL of the cytokine.
Storage & Stability
Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted mouse IL-7 can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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