Recombinant Murine Interleukin-1 alpha (rMu IL-1 alpha) is a potent immuno-modulator, which mediates a wide range of immune and inflammatory responses. Murine IL-1 alpha is a 18 kDa protein containing 156 amino acid residues. It has benn purified using proprietary chromatographic techniques.
Mouse Il1a expressed in E.coli
CAT# CSC-CTK0678-10 (10 μg); CAT# CSC-CTK0678-50 (50 μg)
Greater than 98% as determined by SDS-PAGE and HPLC analyses.
The ED50 as determined by the dose-dependent stimulation of murine D10S cells is ≤ 0.01 ng/ml, corresponding to a specific activity of Greater than 1 x 10^8 units/mg.
Less than 1 EU/μg.
Lyophilized from a concentrated (1 mg/ml), sterile-filtered protein solution in 1x PBS (pH 7.4).
Please centrifuge product before opening the vial. The lyophilized protein should be reconstituted in sterile, ultrapure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rMu IL-1 alpha should be stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.
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