ActoFactor™ Recombinant Mouse Fibroblast growth factor 8

CSC-CTK0666

Recombinant mouse Fibroblast Growth Factor-8 (rHu FGF-8), also known as Androgen-induced growth factor (AIGF), is a heparin binding growth factor, which stimulates the proliferation and activation of cells that express FGF receptors. Creative Bioarrays mouse FGF-8 produced in E. coli is a single, non-glycosylated polypeptide chain of 246 amino acids and a molecular mass of 28.1 kDa. It has been purified by proprietary chromatographic techniques.

Mouse

Mouse Fgf8 expressed in E.coli

28.1 kDa

CAT# CSC-CTK0666-25 (25 μg); CAT# CSC-CTK0666-125 (125 μg)

E.coli

Greater than 95% as determined by SDS-PAGE and HPLC analysis.

The ED50, as determined by the dose-dependent a cell proliferation assay using NR6R-3T3 mouse fibroblast cells, is 40,000 units/mg.

Less than 1 EU/μg.

White (freeze-dried) powder. FGF-8 protein was lyop hilized from a 0.2 μm filtered, concentrated protein solution in PBS (pH 7.4).

Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.