Granulocyte Colony Stimulating Factor (G-CSF) is a potent stimulator of bone marrow cells, especially those of neutrophil lineage. Recombinant Murine G-CSF (rMu G-CSF) is a single, non- glycosylated polypeptide chain of 19.0 kDa containing 179 amino acid residues.
Mouse Csf3 expressed in E.coli
CAT# CSC-CTK0669-10 (10 μg); CAT# CSC-CTK0669-50 (50 μg)
Greater than 95% as determined by SDS-PAGE and RP-HPLC analyses.
Recombinant mouse G-CSF is fully biologically active when compared to a standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells (measured by 3H-thymidine uptake) is 0.01-0.03 ng/ml, corresponding to a specific activity of Greater than 1 x 10^7 units/mg.
Less than 1 EU/μg.
Lyophilized from a sterile-filtered solution with no additives.
Please centrifuge product before opening the vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted murine G-CSF should be used immediately or stored long- term in undiluted working aliquots at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.
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