Neurturin is a disulfide-linked homodimer neurotrophic factor structurally related to GDNF, Artemin, and Persephin. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Neurturin signals through a multicomponent receptor system, composed of RET and one of four GFR-alpha (alpha1-alpha4) receptors. Neurturin promotes the development and survival of sympathetic and sensory neurons by signaling through a receptor system composed of RET and GFRalpha2. The functional form of human Neurturin is a disulfide- linked homodimer, of two 11.8 kDa polypeptide monomers (206 total amino acid residues). Each monomer contains seven conserved cysteine residues, one of which (Cys 69) is used for inter- chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration.
Human NRTN expressed in E.coli
CAT# CSC-CTK0602-10 (10 μg); CAT# CSC-CTK0602-50 (50 μg)
Greater than 98% as determined by SDS-PAGE and HPLC analysis.
Human Neurturin at a concentration of 100 ng/ml will support the survival of 65% of newborn rat sympathetic neurons.
Less than 1 EU/μg.
Lyophilized from a sterile-filtered solution in 10 mM sodium citrate (pH 4).
Please centrifuge product briefly before opening the vial. Reconstitute in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted NTN should be used immediately or stored in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.
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