Neurotrophin-3 (NT-3) is a member of the nerve growth factor (NGF) family of neurotrophins that binds various receptors including Trk, TrkB, TrkC and the low affinity NGF receptor. Neurotrophins are key regulators of neural differentiation and survival (for a review, see 1). Together with other NGF family members NT-4 and BDNF, NT-3 has been described as a survival factor for human embryonic stem cells (hESC). Additionally NT-3 has been used to direct the differentiation of hESC to neural lineages. Bioactive NT-3 is predicted to be a non-covalently linked homodimer. Mature recombinant NT-3 has a predicted molecular mass of approximately 13.6 kDa, with 119 amino acids following cleavage of the signal peptide and proprotein. The amino acid sequence of mature NT-3 is identical in human, mouse and rat.
Human NTF3 expressed in Sf21 insect cells
The DNA sequence encoding the NT-3 prepropeptide3 was inserted in a baculovirus expression vector and expressed in Sf 21 insect cells.
CAT# CSC-CTK0067-5 (5 μg); CAT# CSC-CTK0067-25 (25 μg)
Sf21 insect cells
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
The ED50 of NT-3, as measured by its ability to stimulate proliferation of the TrkB-transfected cell line, BaF-TrkB-BD, is typically 10 - 30 ng/mL.
Endotoxin level is less than 1.0 EU/μg of NT-3, as determined by the LAL method.
Lyophilized from a sterile-filtered solution in 30% acetonitrile plus 0.1% TFA containing 50 μg of bovine serum albumin per 1 μg of NT-3.
It is recommended that a stock solution at a concentration of no less than 10 μg/mL be prepared in sterile phosphate buffered saline containing at least 0.1% human or bovine serum albumin.
Storage & Stability
The lyophilized sample is stable for up to 1 year at -20°C to -70°C. Reconstituted NT-3 can be stored under sterile conditions at 2 - 8°C for 1 month or at -20°C to -70°C for 3 months in a manual defrost freezer without detectable loss of acti vity. Avoid repeated freezing and thawing.
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