Pro-NGF is the pro-form of the neurotrophin nerve growth factor. Like the mature protein Pro- NGF is characterized by the cysteine knot motif consisting of three cysteine bridges. The protein predominantly exists as a non-covalently linked homodimer. Recombinant human Pro-NGF produced in E. coli is a non-glycosylated polypeptide chain of 222 amino acids and a molecular mass of 49,738 Dalton.
Human NGF expressed in E.coli
CAT# CSC-CTK0480-10 (10 μg); CAT# CSC-CTK0480-50 (50 μg)
Greater than 98% as determined by SDS-PAGE and RP-HPLC analysis.
The activity of the protein can by measured by its stimulating effect on the proliferation of TF1 cells (Chevalier et al. 1994, Blood 83, 1479-85). EC50 = 130 ± 30 pM (TF1 cell assay).
Less than 1 EU/μg.
The protein solution (1.2 mg/ml; 10 μg/9 μl) contai ns 50 mM sodium phosphate buffer (pH 7.2) and 150 mM sodium chloride.
Please centrifuge product briefly before opening vial. This protein solution can be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The protein solution, though stable at 15°C for up to 3 weeks, is best stored desiccated at 4°C. It should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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