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ActoFactor™ Recombinant Human Lectin, galactoside-binding, soluble, 14

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Cat.No.
CSC-CTK0587
Description
Galectins are a family of animal lectins with an affinity for beta-galactosides. This family has at least 14 identified members. Galectins share similarities in the CRD (the carbohydrate recognition domain). Galectins are synthesized as cytosolic proteins. Though localized principally in the cytoplasm and lacking a classical signal peptide, galectins can also be stimulated to secretion by non-classical pathways or alternatively targeted to the nucleus. Galectins are involved in modulating cell-cell and cell-matrix interactions. Galectin-14 is a member of the galectin family of carbohydrate binding proteins. The Galectin-4 gene is predominantly intracellularly in the placenta and eosinophils and is released by eosinophils following allergen stimulation. Galectin-4 may be involved in the development of allergic inflammation. Human recombinant Galectin-14 produced in E. coli is fused with a 23 amino acid His-tag at the N-terminus. It is a single, non-glycosylated polypeptide chain of 162 amino acids (1-139 a.a.) and a molecular mass of 18.5 kDa. It has been purified by proprietary chromatographic techniques.
Organism
Human
Product Overview
Human LGALS14 expressed in E.coli
Molecular Mass
18.5 kDa
Size
CAT# CSC-CTK0587-5 (5 μg); CAT# CSC-CTK0587-25 (25 μg)
Expression System
E.coli
Purity
Greater than 90% as determined by SDS-PAGE analysis.
Activity
N/A
Endotoxin Level
Less than 1 EU/μg.
Formulation
Sterile filtered, colorless solution (0.5 mg/ml) co ntaining 20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1 M NaCl and 1 mM DTT.
Reconstitution
Please centrifuge product briefly before opening vial. The dissolved protein can be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
Store at 4°C if entire vial will be used within a week. Store frozen at -20°C for longer periods of time. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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