The IL-2 receptor system consists of three non-covalently linked subunits termed IL-2R alpha, IL- 2R beta, and IL-2R gamma. The IL-2R alpha is a type I transmembrane protein consisting of a 219 amino acid (a.a.) extracellular domain, a 19 a.a. transmembrane domain and a 13 a.a. intracellular domain, which is not involved in the transduction of IL-2 signals. Proteolytic processing of IL-2R alpha releases the entire extracellular domain of IL-2R alpha thereby generating a 219 a.a. soluble protein called soluble IL-2R alpha (sIL-2R alpha). The homodimeric form binds IL-2 (KD=10 mM) and facilitates IL-2 signaling. The secreted sIL-2R alpha is expressed on leukemia cells, lymphoma cells, newly activated T and B cells, as well as on approximately 10% of NK cells. Recombinant human sIL-2R alpha is a 24.8 kDa protein containing 219 amino acid residues consisting of only the extracellular domain of IL-2R-alpha. Due to glycosylation, IL-2R-alpha has an approximate molecular weight of 31 kDa based on SDS-PAGE gel and mass spectrometry.
Human IL2RA expressed in (BTI-Tn-5B1-4) Hi-5 insect cells
CAT# CSC-CTK0304-25 (25 μg); CAT# CSC-CTK0304-125 (125 μg)
(BTI-Tn-5B1-4) Hi-5 insect cells
Greater than 98% as determined by SDS-PAGE and HPLC analyses.
Determined by its ability to increase the proliferation effect of IL-2 in mouse CTLL-2 cells. In the presence of 1 ng/ml of recombinant IL-2, the expected ED50 for this effect is between 0.5-1.5 μg/ml.
Less than 1 EU/μg.
Sterile filtered through a 0.2 micron filter and lyophilized from 1x PBS.
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile 1x PBS (pH 7.2) to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored dessicated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at –20°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.
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