ActoFactor™ Recombinant Human Interferon, gamma

CSC-CTK0191

IFN-gamma, a type II interferon and an acid-labile interferon, is mainly produced by CD4 and CD8 T-lymphocytes and natural killer cells activated by antigens, mitogens or alloantigens. IFN- gamma shows no significant homology with IFN-beta or IFN-alpha. IFN-gamma has antiviral and antiparasitic activities and exhibits also immunoregulatory and proinflammatory activities. IFN- gamma induces cytokine production and promotes antigen presentation by up regulating the expression of MHC proteins as well as other antigen proteins. In contrast to other interferons, which are mostly antiviral, the main activity of IFN-gamma seems to be immunomodulatory. Human IFN-gamma is species-specific and is only biologically active in human and primate cells. Recombinant human IFN-gamma produced in plant is a 18.8 kDa protein containing 143 amino acid residues and contains a 16 a.a. histidine-based tag for a total length of 159 a.a. As a result of glycosylation, the recombinant protein migrates as two bands with an apparent molecular mass of 25 and 27 kDa in SDS-PAGE.

Human

Human IFNG expressed in Endosperm tissue of barley grain (Hordeum vulgare)

18.8 kDa

CAT# CSC-CTK0191-100 (100 μg); CAT# CSC-CTK0191-500 (500 μg)

Endosperm tissue of barley grain (Hordeum vulgare)

Greater than 95% as determined by SDS-PAGE analysis.

The ED50 as determined by the dose-dependent cytostatic effects of serial dilutions of IFN- gamma in the presence of TNF-alpha on HT-29 (human carcinoma) cells is 0.12 ng/ml corresponding to a specific activity of 8.5 x 10^6 units/mg. The optimal concentration should be determined for specific applications and cell lines.

Less than 0.05 EU/μg.

Sterile filtered through a 0.2 μm filter. Lyophilized from PBS (pH 7.4).

Please centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

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