Interferon-g (IFN-g), also known as Type II interferon or immune interferon, is a highly species-specific pleiotropic cytokine produced primarily by T and NK cells. Natural mature IFN-g exists as non-covalently-linked homodimers with different levels of glycosylation. IFN-g exerts antiviral, antiproliferative, immunoregulatory and proinflammatory activities. It induces the production of cytokines, upregulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. IFN-g also augments Th1 cell expansion and may be required for Th1 cell differentiation. IFN-g binds to a heterodimer cell surface receptor that when stimulated activates the JAK-STAT pathway. Recombinant human IFN-g is a 17 kDa protein containing 144 amino acid residues.
Human IFNG expressed in E.coli
A DNA sequence encoding the mature human IFN-g protein was expressed in E. coli.
CAT# CSC-CTK0047-100 (100 μg); CAT# CSC-CTK0047-500 (500 μg)
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity was determined by measuring the antiviral effect using HeLa cells infected with EMC virus and the ED50 using this assay was typically 8-15 units/mL or 0.8-1.5 ng/mL, giving a specific activity of approximately 1 x 10^7 units/mg protein.
Endotoxin level is less than 0.1 ng per mg of the cytokine, as determined by the LAL method.
Lyophilized from a 0.2 μm filtered solution in PBS containing 50 mg of bovine serum albumin per 1 mg of cytokine.
It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 10 mg/ml of the cytokine.
Storage & Stability
The lyophilized sample is stable for greater than six months at -20°C to -70°C. Reconstituted IFN-gcan be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freezing and thawing.
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