ICAMs are members of the Ig superfamily of calcium-independent transmembrane glycoproteins. CD54 (ICAM-1) is a ligand for lymphocyte function-associated (LFA) and Mac-1 integrins and the major human rhinovirus receptor. The primary function of CD54 (ICAM-1) is to provide adhesion between endothelial cells and leukocytes after stress or injury. The human CD54 (ICAM-1) gene codes for a 505 amino acid transmembrane glycoprotein containing a 29 amino acid cytoplasmic domain, a 23 amino acid transmembrane domain, and a 453 amino acid extracellular domain. Recombinant human CD54 (ICAM-1) is a 49.5 kDa glycoprotein comprising the extracellular domain (453 amino acid residues) of CD54 (ICAM-1). Monomeric glycosylated CD54 (ICAM-1) migrates at an apparent molecular weight of approximately 72.0- 80.0 kDa by SDS-PAGE analysis under reducing conditions.
Human ICAM1 expressed in CHO cells
CAT# CSC-CTK0309-50 (50 μg); CAT# CSC-CTK0309-250 (250 μg)
Greater than 98% as determined by SDS-PAGE and HPLC analysis.
ICAM-1 supported the adhesion of more than 50% PMA-stimulated HSB2 cells to plates coated at 25 μg/ml ICAM-1 concentration.
Less than 1 EU/μg.
Lyophilized from a sterile-filtered (0.2 micron filter) solution in 10 mM sodium phosphate (pH 7.5).
Please centrifuge product before opening vial. The lyophilized protein should be reconstituted with distilled, sterile water to a concentration of 0.1 - 1.0 mg/ml. For further dilution, carrier protein (0.1% BSA or HSA) should be added to avoid loss of bioactivity.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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