GDF-15 belongs to the TGF-β cytokine family whose members play an important role during prenatal development and postnatal growth, remodeling and maintenance of a variety of tissues and organs. GDF-15 is expressed predominantly in placenta and to a much lesser extent in various other tissues. The presence of GDF-15 in amniotic fluid and its elevated levels in the sera of pregnant women suggest a role for GDF-15 in gestation and embryonic development. GDF- 15 generally exerts tumor suppressive activities and is one of the predominant factors produced and secreted in response to activation of the p53 pathway. Interestingly, the serum level of GDF- 15 is positively correlated with neoplastic progression of several tumor types, including certain colorectal, pancreatic, and prostate cancers. Human GDF-15/MIC-1 is a disulfide linked homodimeric protein consisting of two 114 amino-acid polypeptide chains.
Human GDF15 expressed in Cell culture (human cell line)
CAT# CSC-CTK0539-20 (20 μg); CAT# CSC-CTK0539-100 (100 μg)
Cell culture (human cell line)
Greater than 95% as determined by SDS-PAGE and HPLC analysis.
Determined by its ability to inhibit alkaline phosphatase activity in differentiating MC3T3/E1 osetoblast cells. The expected ED50 for this effect is 1.0-3.0 ng/ml.
Less than 1 EU/μg.
Lyophilized (freeze-dried) powder. Lyophilized from a st erile-filtered (0.2 micron filter) solution in 10 mM sodium citrate (pH 3.0).
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu GDF-15 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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