MEC is a secreted CC chemokine expressed primarily by epithelial cells of the bronchioles, salivary gland, mammary gland and colon. MEC signals through the CCR10 receptor and chemoattracts resting CD4, CD8 T-cells and eosinophils. MEC contains six cysteines including the four highly conserved cysteine residues present in CC chemokines. Recombinant human MEC is a 12.3 kDa protein containing 108 amino acid residues.
Human CCL28 expressed in E.coli
CAT# CSC-CTK0577-20 (20 μg); CAT# CSC-CTK0577-100 (100 μg)
Greater than 98% as determined by SDS-PAGE and HPLC analysis.
Determined by its ability to chemoattract human lymphocytes using a concentration range of 1.0-10.0 ng/ml.
Less than 1 EU/μg.
Lyophilized from a sterile-filtered solution in 10 mM acetic acid.
Please centrifuge product briefly before opening the vial. Reconstitute in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted MEC should be used immediately or stored in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.
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