Brain-derived neurotrophic factor (BDNF) is a member of the nerve growth factor family of proteins. It binds with high affinity to the TrkB kinase receptor. BDNF has been used to direct human embryonic stem cells (hESC) to neural lineages, and, together with neurotrophin-3 (NT-3) and NT-4, it has been described as a survival factor for hESC1. Mature recombinant BDNF has a predicted molecular mass of approximately 13.6 kDa, with 119 amino acids. The amino acid sequence of mature BDNF is identical in all mammals examined.
Human BDNF expressed in Sf21 insect cells
The DNA sequence encoding the BDNF prepropeptide2 was inserted in a baculovirus expression vector and expressed in Sf 21 insect cells.
CAT# CSC-CTK0006-5 (5 μg); CAT# CSC-CTK0006-25 (25 μg)
Sf21 insect cells
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
The ED50 of BDNF is typically 3 - 10 ng/mL, as measured by its ability to stimulate proliferation of the TrkB transfected cell line, Baf-TrkB.
Endotoxin level is less than 1.0 EU/μg.
Lyophilized from a sterile-filtered solution in 0.1 M Sodium Citrate (pH 3.0) and 0.3 M sodium chloride containing 50 μg of bovine serum albumin per 1 μg of BDNF.
It is recommended that sterile It is recommended that sterile phosphate-buffered saline containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution at no less than 10 μg/mL.
Storage & Stability
The lyophilized powder is stable for upto 6 months at -20°C to -70°C. Reconstituted BDNF can be stored under sterile conditions at 2 - 8°C for 1 month or at -20°C to -70°C for 3 months in a manual defrost freezer without detectable loss of acti vity. Avoid repeated freezing and thawing.
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