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Immortalized Human Urethral Epithelial Cells
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Cat.No.
CSC-I9275L
Description
Primary human urethral epithelial cells were isolated and immortalized with the recombinant retrovirus vector pLXSN containing the HPV16 E6E7 oncogenes to achieve the generation of the Immortalized Human Urethral Epithelial Cells. The cells have telometric activity, and expresses cytokeratins 13 and 16 markers and the ASGP-R (asialoglycoprotein) receptor. The immortalized cells retained the primary phenotypes when compared to the primary cells. Immortalized Human Urethral Epithelial Cells are an unlimited source of tissue culture urethral epithelial cells for downstream applications. It may be used to study the urethral epithelium and possible investigations in pathogenesis in the urethra.
Species
Homo sapiens
Source
Membranous urethral tissue explants
Culture Properties
Adherent
Morphology
Epithelial-like
Immortalization Method
Serial passaging and transduction with recombinant retrovirus vector pLXSN containing the HPV16 E6E7 oncogenes
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
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Quality Control
Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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