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Immortalized Human Trophoblast Cells-hTERT (Sw.71)
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Cat.No.
CSC-I9150L
Description
The Immortalized Human Trophoblast Cells - hTERT (Sw.71) were derived from 7-week first trimester placenta isolated from normal pregnancy and were virally infected with hTERT. The immortalized cells retained the same morphology as their primary counterparts. It has the abilities to spontaneously fuse as occasional multi-nucleated cells clusters may form, and can secrete cytokines (IL-1β, IL-6, IL-8, Monocyte Chemotactic Protein-1 (MCP-1), GRO (CXCR2), GRO-α (CXCL1), Intercellular Adhesion Molecule-1 (ICAM-1), Osteoprotegerin (OPG), Tissue Inhibitor of Metalloproteinase-1 (TIMP-1), TIMP-2 and VEGF). Cytokeratin 7 and vimentin markers are expressed. The population of cells is determined to be free of immune cells due to the absence of CD45 and CD68, and free of fibroblast cells due to the lack of fibroblast specific antigen. The Sw.71 cells are a valuable tool for trophoblast research.
Species
Homo sapiens
Source
Placenta
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
Serial passaging and transduction with retroviruses expressing hTERT from the supernatant of pA317-hTERT cell line
Markers
Cytokeratin 7 and Vimentin
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CIK-HT013 HT® Lenti-hTERT Immortalization Kit
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
1) hTERT activity was analyzed via TRAPeze ELISA Detection Kit;
2) Human Cytokine Array 3.1 and III was used to determine cytokine profile;
3) Immunocytochemistry was utilized for determining the presence of cell markers.
2) Human Cytokine Array 3.1 and III was used to determine cytokine profile;
3) Immunocytochemistry was utilized for determining the presence of cell markers.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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