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Immortalized Human Tonsillar Epithelial Cells (E6/E7 HTE)
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Cat.No.
CSC-I9343L
Description
The Immortalized Human Tonsillar Epithelial Cells (E6/E7 HTE) were isolated from routine bilateral tonsillectomy patients and were immortalized by transfection with retroviral vectors containing HPV-16 E6 and E7. When compared to the primary cells, these immortalized cells retain the original morphological characteristics. The Immortalized Human Tonsillar Epithelial Cell line (E6/E7 HTE) is a suitable model for studies in carcinogenesis as it allows in vitro studies of epithelial cancer formation and the role of HPV in conversion of a normal cell to cancer cell.
Species
Homo sapiens
Source
Tonsil
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
Transfection with retroviral vectors containing HPV16-E6 and E7
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
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CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
1) R-PCR quantitation of E6 and E7 oncogenes;
2) Anchorage-independent growth assay.
2) Anchorage-independent growth assay.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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