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Immortalized Human Oral Mucosal Epithelial Cells (hTERT-OME)
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Cat.No.
CSC-I9117L
Description
Primary oral epithelial cells were derived from patients undergoing removal of impacted teeth and were immortalized with the retroviral transfection of the pLXSN-hTERT vector to establish the Immortalized Human Oral Mucosal Epithelial Cells (hTERT-OME). The cells are also considered to be oral keratinocytes and have maintained their stem cell properties. hTERT-OME cells are a valuable tool for research in the biology of oral epithelial cells.
Species
Homo sapiens
Source
Gingival mucosa
Culture Properties
Adherent
Morphology
Spindle-shaped
Immortalization Method
Transfection with the retroviral vector (pLXSN–hTERT)
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
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CIK-HT013 HT® Lenti-hTERT Immortalization Kit
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
1) Immunocytochemistry showed the cells expressed cytokeratin and vimentin;
2) Western blot and RT-PCR detected the presence of hTERT
2) Western blot and RT-PCR detected the presence of hTERT
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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