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Immortalized Human Nasopharyngeal Epithelial Cells (NPEC1/Bmi1)
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Cat.No.
CSC-I9352L
Description
The Immortalized Human Nasopharyngeal Epithelial Cells (NPEC1/Bmi1) are collected from the biopsy of a patient diagnosed with nonmalignant nasal disease and were stably transfected with the retroviral vector pMSCV-Bmi-1. The immortalized cells maintain a normal p53 checkpoint which is a normal DNA damage response. In addition, the NPEC1/Bmi1 cells are also unlikely to have other undefined genetic lesions in comparison to other tumor-derived cells immortalized with viral oncogenes. Therefore, the Immortalized Human Nasopharyngeal Epithelial Cells (NPEC1/Bmi1) are considered the first cellular proto-oncogene immortalized nasopharyngeal epithelial cells. These cells are useful in serving as a cell model system for studying the mechanisms involved in the tumorigenesis of nasopharyngeal carcinoma using defined genetic elements.
Species
Homo sapiens
Source
Nasopharynx
Culture Properties
Adherent
Morphology
Cobblestone-like
Immortalization Method
Transfection with retroviral vector pMSCV-Bmi-1.
Markers
Bmi-1, BUB1, HEC1, KI67
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
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CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
1) Puromycin selection with 0.5 μg/mL final concentration;
2) Immunohistochemical analysis using cytokeratin;
3) Immunoblot and immunostaining analysis using anti-Bmi-1 antibodies
2) Immunohistochemical analysis using cytokeratin;
3) Immunoblot and immunostaining analysis using anti-Bmi-1 antibodies
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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