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Immortalized Human Lung Fibroblast-hTERT

  • Specification
  • Recommended Products
Cat.No.
CSC-I9012L
Species
Homo sapiens
Source
Lung
Culture Properties
Adherent
Morphology
Fibroblast
Immortalization Method
Serial passaging and transduction with recombinant lentiviruses carrying hTERT gene
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CSC-C4091X Human Lung Fibroblasts
CIK-HT013 HT® Lenti-hTERT Immortalization Kit
Quality Control
Real Time PCR was used to quantify hTERT gene expression in immortalized cell line.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

  • Q & A
  • Customer Review
Q:
How to pass on the cells?
A:

If the customer receives 2 mL small tube cells, after receiving the cells, spray the whole tube with 75% alcohol to sterilize it and put it into the ultra-clean table or safety cabinet, strictly aseptic operation. Transfer the small tube cells to the T25 culture flasks or 6 cm Petri dishes, add about 5 mL of complete medium to mix well, and then put it into the incubator to check the density of the cells after overnight cultivation: if the density is not more than 80%, change the liquid to continue the cultivation, and then pass on the cells as appropriate or If the density is not more than 80%, change the liquid and continue to culture, passaging or freezing as appropriate. If the density exceeds 80%, the cells can be passaged directly (same method as above).

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Average Rating: 5.0    |    1 Scientist has reviewed this product

Wonderful

Upon receipt of the cell products, I carried out a check of the product numbers and packaging and they were all correct.

10 Feb 2021


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