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Immortalized Human Hepatocytes-HPV and hTERT

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Cat.No.
CSC-I9340L
Description
Human hepatocytes plays an important role in life science research for evaluation of key human-specific drug properties, such as of drugs interactions, toxicity tests and metabolism reactions. Since primary cells are often hard to propagate in culture. Creative Bioarray offers immortalized human hepatocytes that allows the cells to escape senescence crisis and help further research needs by extending the passage ability that primary cells lacks. These cells are ready-to-use for applications in research and developmental viral infections, screening, co-cultures and transfections.
Species
Homo sapiens
Source
Liver
Culture Properties
Adherent
Immortalization Method
Serial passaging and transduction with lentiviruses carrying Human papillomavirus E6/E7 protein and hTERT gene.
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CSC-C1495 Human Hepatocytes
CIK-HT018 HT® Lenti-HPV-16 E6/E7 Immortalization Kit
CIK-HT013 HT® Lenti-hTERT Immortalization Kit
Quality Control
Real Time PCR was used to quantify HPV E6/E7 and hTERT gene expression in immortalized cell line.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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