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Immortalized Human Fallopian Tube Secretory Epithelial Cells (FT33-shp53-R24C)
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Cat.No.
CSC-I9204L
Description
The fallopian tube epithelium is lined by both ciliated and secretory epithelial cells. Recently, the fallopian tube epithelial secretory epithelial cells (FTSEC) are proposed to be the precursor tissue for high grade serous ovarian cancer (HGSC), the most lethal gynecological malignancies. The Immortalized Human Fallopian Tube Epithelial Secretory Cells (FT33-shp53-R24C) maintains its Müllerian phenotype and continues to express lineage-specific markers PAX8 and CK7, thus providing a valuable tool for studies of normal fallopian tube epithelium and early-stage development of HGSC.
Species
Homo sapiens
Source
Fimbriae of fallopian tube
Culture Properties
Adherent
Morphology
Cobblestone-like
Immortalization Method
Serial passaging and transduction with retroviruses stably expressing hTERT, p53 shRNA and CDK4R24Cmutant gene
Markers
PAX8, CK7
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
CIK-HT013 HT® Lenti-hTERT Immortalization Kit
CIK-HT013 HT® Lenti-hTERT Immortalization Kit
Quality Control
1) Western blot and RT-PCR were used to evaluate the hTERT and CDK4 transgene expression;
2) CDK4 functional studies was performed to assess the hyperphosphorylation of pRb at Ser780;
3) Immunofluorescent staining was used to determine PAX8 expression.
2) CDK4 functional studies was performed to assess the hyperphosphorylation of pRb at Ser780;
3) Immunofluorescent staining was used to determine PAX8 expression.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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