 
            Immortalized Human Dermal Microvascular Endothelial Cells
Cat.No.: CSC-I9095L
Species: homo sapiens
Source: Skin
Morphology: Polygonal
Culture Properties: Adherent
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- Q & A
- Customer Review
Cat.No.
CSC-I9095L
Description
Immortalized Human Dermal Microvascular Endothelial Cells provided by Creative Bioarray have been developed by immortalizing primary human dermal endothelial cells with SV40 Large T antigen. Immortalized cells were controlled passaging side by side with the primary cells. Primary cells go into senescence after the 3rd passage while the SV40‐tranduced cells go beyond 30 passages.
Species
                                homo sapiens
                            Source
                                Skin
                            Recommended Medium
                                SuperCult® Immortalized Human Dermal Microvascular Endothelial Cell Medium (Cat No.: CM-I9095L)
                            Freezing Medium
                                Complete medium supplemented with 10% (v/v) DMSO
                            Culture Properties
                                Adherent
                            Morphology
                                Polygonal
                            Immortalization Method
                                SV40 large T antigen
                            Markers
                                Factor VIII- related antigen
                            Application
                                For Research Use Only
                            Growth Properties
                                Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
                            Storage
                                Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
                            Note: Never can cells be kept at -20 °C.
Shipping
                                Dry Ice.
                            Recommended Products
                                CSC-C4042X  Human Dermal Microvascular Endothelial Cells (HDMEC) juvenile foreskin
CIK-HT013 HT® Lenti-hTERT Immortalization Kit
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
                            CIK-HT013 HT® Lenti-hTERT Immortalization Kit
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
                                Real Time PCR was used to quantify SV40T gene expression in immortalized cell line.
free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations
                            free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations
BioSafety Level
                                II
                            Citation Guidance
                        
                            If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
                            
                                If your paper has been published, please click here
                                to submit the PubMed ID of your paper to get a coupon.
                            
                        
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