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Immortalized Human Cystic Fibrosis Tracheobronchial Epithelial Cells (CFT1)
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Cat.No.
CSC-I9334L
Description
Cystic fibrosis (CF) is a genetic disease affecting the exocrine glands in which production of abnormal thick mucus causing build up in the bronchi, pancreatic ducts, and the intestines which may lead to blockages and eventually resulting in respiratory infection. The Immortalized Human Cystic Fibrosis Tracheobronchial Epithelial Cells (CFT1) is an applicablein vitromodel for further research in CF which exhibits differentiated phenotypes in ion transport and have a decrease regulation of Cl- ion channel. The primary cells were derived from a 24 year-old, male donor which the history of meconium ileus at birth, pancreatic insufficiency, elevated sweat Cl-, bronchiectatic lung disease with chronicStaphylococcus aureusandPseudomonas aeruginosainfection, and was determined to be homozygous for phenylalanine-508 deletion in the CF transmembrane conductance regulator (CFTR) gene. Immortalization with HPV-18 E6/E7 established a continuous cell line for experimental studies for the CFTR gene and function.
Species
Homo sapiens
Source
Trachea
Culture Properties
Adherent
Morphology
Epithelial-like
Immortalization Method
Transfection with the pUC19 plasmid encoding intact HPV-18 E6 and E7 gene
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
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Quality Control
Immunoblot used to determine the expression of CFTR, and presence of HPV-18 E6/E7 gene.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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