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Immortalized Human Corneal Keratinocytes (IHCK)
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Cat.No.
CSC-I9231L
Description
Corneal Keratinocytes play a major role in maintaining the balance of stromal substances and preserving the corneal transparency. Established using the HPV16 E6/E7 genes, The Immortalized Human Corneal Keratinocytes (IHCK) not only retains its authentic cell biomarkers (CK12, CK19), it also displays fairly disomic cytogenetic status at chromosomes 1, 8, 10 and 18, which is an indication of normal diploid chromosomal status.Together with the Immortalized Human Corneal Stromal Fibroblasts and the Immortalized Human Corneal Endothelial cells isolated from the same donor and immortalized using the same platform, this uniform model system presents a valuable tool for studies involving cornea homeostasis and ophthalmologic diseases.
Species
Homo sapiens
Source
corneoscleral rim
Culture Properties
Adherent
Morphology
polygonal
Immortalization Method
Serial passaging and transduction with retroviruses carrying human papillomavirus type 16 E6/E7 gene
Markers
CK19, CK12
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CIK-HT013 HT® Lenti-hTERT Immortalization Kit
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
1) RT-PCR was used to evaluate the transgene expression of HPV16 E6/E7;
2) Indirect immunofluorescence was performed to detect the cell specific marker expression CK12, CK19 and ABCG2;
3) FISH was used to examine the cytogenetic alterations in the immortalized cells.
2) Indirect immunofluorescence was performed to detect the cell specific marker expression CK12, CK19 and ABCG2;
3) FISH was used to examine the cytogenetic alterations in the immortalized cells.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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