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Immortalized Human Cervical Keratinocytes (16E6/E7 HCK)
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Cat.No.
CSC-I9333L
Description
The Immortalized Human Cervical Keratinocytes (16E6/E7 HCK) were derived from normal human ectocervix cells from surgical specimens and were immortalized by transfection with retrovirus expressing HPV-16 E7 and E6. These immortalized cells are suitable as a model for carcinogenesis and cellular senescence as the expression of HPV-E6 and E7 proteins, and inactivation of both p53 and Rb, extend the lifespan of cells past a first critical period when the normal cells senesce. Having telomere restoration ability, the Immortalized Human Cervical Keratinocytes (16E6/E7 HCK) are also useful for studies focusing on understanding the role of telomere loss and restoration in the development of HPV-associated human cancers.
Species
Homo sapiens
Source
Cervix
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
Transfection with retrovirus expressing HPV-16 E7 and HPV-16 E6
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CIK-HT013 HT® Lenti-hTERT Immortalization Kit
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
1) FACS with propidium iodide staining;
2) RIPs to detect HPV-16 E6 and E7.
2) RIPs to detect HPV-16 E6 and E7.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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