Immortalized Human Cardiac Fibroblasts

Cat.No.: CSC-I9134L

Species: homo sapiens

Source: Ventricles (Adult Heart)

Morphology: Multipolar

Culture Properties: Adherent

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Cat.No.

CSC-I9134L

Description

Human Cardiac Fibroblast is an important heart component that provides structural support for cardiomyoctes. They synthesize extracellular matrix, growth factors and cytokines during growth. In addition, they also interact with myocardial responses to injury and pathophysiological conditions such as scar formation at sites of myocardial infarction, cardiac fibrosis and cardiac hypertrophy. These cells are ideal models for study in cardiac matrix response modeled by physiological and pathological stressors. Immortalized Human Cardiac Fibroblasts-SV40 were derived from Primary Human Cardiac Fibroblasts transduced with a lentiviral expression vector containing the SV40T gene. The cell line was continuously cultured for more than 20 passages without showing signs of growth retardation or replicative senescence.

Species

homo sapiens

Source

Ventricles (Adult Heart)

Recommended Medium

SuperCult® Immortalized Human Cardiac Fibroblast Medium (Cat No.: CM-I9134L)

Freezing Medium

Complete medium supplemented with 10% (v/v) DMSO

Culture Properties

Adherent

Morphology

Multipolar

Immortalization Method

SV40 large T antigen

Application

For Research Use Only

Growth Properties

Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂.

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Note: Never can cells be kept at -20 °C.

Shipping

Dry Ice.

Recommended Products

CSC-7818W Human Cardiac Fibroblasts
CIK-HT013 HT® Lenti-hTERT Immortalization Kit
CIK-HT003 HT® Lenti-SV40T Immortalization Kit

Quality Control

Real Time PCR was used to quantify SV40T gene expression in immortalized cell line. Free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations.

BioSafety Level

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.