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Immortalized Human Annulus Fibrosus Sheet Cells
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Cat.No.
CSC-I9316L
Description
Immortalized Human Annulus Fibrosus Sheet Cells were derived from healthy disc materials from patients and were immortalized via retroviral transduction carrying the SV40LTAg and hTERT genes. The cells retained its primary phenotype and express the COL1A1, COL5A1, COL12A1 and SFRP-2 markers. It is able to form sheets, unlike the Immortalized Human Annulus Fibrosus Non-Sheet Cells. The Immortalized Human Annulus Fibrosus Sheet Cells may be used to study the formation of cell sheets, and insight on the biology of the annulus fibrosus and disc pathology.
Species
Homo sapiens
Source
Disc Tissue
Culture Properties
Adherent
Morphology
Fibroblast-like
Immortalization Method
Transduction with retroviral particles expressing SV40LTAg (pBABE-hygro) and hTERT (pBMN-IRES-NEO)
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Quality Control
PCR used to detect markers
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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