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Immortalized Human Adipose-derived Stromal Cells-Bmi-1/hTERT
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Cat.No.
CSC-I9090L
Description
With its ability to maintain multipotency, mesenchymal stem cells provide new venues for cell-based therapy in restoration of damaged or diseased tissue. Most clinical studies utilize bone marrow-derived stem cells, however adipose-derived stromal cells (ASCs) is emerging as an alternative source for their ease of harvesting. The Immortalized Adipose-derived Stromal Cells- Bmi-1/hTERT is derived from human ASCs that have maintained their primary phenotypes and their ability to respond to osteogenic and adipogenic inductions. After 3 weeks of induced osteogenic differentiation, the immortalized ASCs show nuclear translocation of RUNX2, upregulation of alkaline phosphatase and calcium deposition. Furthermore, the immortalized ASCs-Bmi-1/hTERT exhibit normal karyotype and has been shown to exceed 140 population doublings, making these cells suitable for studies in regenerative medicine.
Species
Homo sapiens
Source
Adipose Tissue
Culture Properties
Adherent
Immortalization Method
Serial passaging and transduction with recombinant lentiviruses carrying mouse Bmi-1 (pLOX-CWBmi1) [Ref. seq.: NM_007552.4] and hTERT (pLOX-TERT-iresTK) [Ref.seq.: NM_198253.2] gene
Markers
CD44, CD73, CD90 and CD105
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CIK-HT013 HT® Lenti-hTERT Immortalization Kit
Quality Control
1. Real Time PCR, immunofluorescence, and telemorase activity was used to quantify Bmi-1 and/or hTERT gene expression in the immortalized cell line.
2. Culture tested negative for mycoplasma
2. Culture tested negative for mycoplasma
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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