Canine Dermal Fibroblasts-Adult

Canine Dermal Fibroblasts (CDFs) are a fibroblastic cell line derived from the dermis of healthy adult dogs (Canis lupus familiaris). As the most abundant stromal cell type in the skin, fibroblasts are major contributors to the synthesis of the ECM and influence the architecture and repair of tissues. As expected of fibroblasts, canine dermal fibroblasts have the spindle-shaped adherent morphology of fibroblasts, and will form aligned bundles of cells, or whorls, upon culture surfaces. Similar to fibroblasts from different species, canine dermal fibroblasts proliferate effectively in DMEM or DMEM/F12 with FBS and demonstrate a doubling time between 24-48 hours but their growth rates show variation depending on the donor source and passage level.

Canine dermal fibroblasts produce various ECM components such as collagens I and III, fibronectin, elastin, and glycosaminoglycans while also responding to multiple cytokines and growth factors. Furthermore, canine dermal fibroblasts can be readily induced to differentiate to α-SMA-positive myofibroblasts in response to TGF-β. As such, they are an ideal cell system to study fibrosis, scarring and contractile wound-healing in vitro. Additionally, canine dermal fibroblasts will produce inflammatory cytokines, chemokines, and other mediators such as IL-6, IL-8, and MIP-1α upon stimulation. This allows CDFs to be used in a variety of dermatologic immunology and infection studies. As CDFs have been isolated directly from canine skin tissue and have not been transformed, they maintain many of the physiologically relevant properties of skin tissue. For this reason, CDFs have been used in many applications including veterinary dermatology, wound healing, toxicology and testing of biomaterials, and mechanistic studies of cellular senescence and cellular stress responses.