BC-3C

Cat.No.: CSC-C0482

Species: Homo sapiens (Human)

Source: Bladder

Morphology: epitheloid adherent cells growing adherently in monolayers and in multilayer foci

Culture Properties: monolayer

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Cat.No.

CSC-C0482

Description

Derived from the invasive solid transitional cell carcinoma of the bladder of a 82-year-old Caucasian woman (grade IV, stage C); cell growth described as being inhibited by EGF

Species

Homo sapiens (Human)

Source

Bladder

Recommended Medium

SuperCult® McCoys 5a Medium and 10% h.i. FBS.

Culture Properties

monolayer

Morphology

epitheloid adherent cells growing adherently in monolayers and in multilayer foci

Karyotype

Human flat-moded hypertriploid karyotype with 10% polyploidy - 65-72<3n>XX, -X, -2, -4, +9, +9, -11, -13, +14, +16, -17, -17, -18, +19, +20, +20, +2-3mar, add(2)(p1?), del(3)(q2?2), der(5;7)(q10;q10), del(9)(p2?2p2?3), add(12)(q24) - sideline with del(2)(

Disease

Bladder Carcinoma

Quality Control

Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Synonyms

BC3c

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.