Immortalized Human Pancreatic Islet-Derived Precursor Cells-SV40

Analysis of MicroRNA Signature Differentially Expressed in Pancreatic Islet Cells Treated with Pancreatic Cancer-Derived Exosomes

PC patients often develop insulin resistance or DM before diagnosis, highlighting PC-DM as a potential detection platform. Here, Kim et al. treated immortalized human pancreatic islet-derived precursor cells (hIPCs) with PC-derived exosomes (from 50 patients) and healthy-derived exosomes (from 50 individuals) and assessed miRNA expression changes using next-generation sequencing and bioinformatic analysis.

In the expression analysis, 2656 miRNA markers were used. The heatmap of normalized miRNA expression from three specimens and the scatter plot from principal component analysis (PCA) are shown in Figure 1. Unsupervised clustering analysis revealed that Control and Healthy clustered together, while Cancer was distant from both, indicating distinct expression profiles. The results of the differential expression analysis (DEmiRNA1 and DEmiRNA2) are shown in Figure 2. DEmiRNA1 (Cancer vs. Control) identified 13 upregulated and 16 downregulated miRNAs. DEmiRNA2 (Healthy vs. Control) identified 14 upregulated and 7 downregulated miRNAs. Among these, 1 miRNA was upregulated in both DEmiRNA1 and DEmiRNA2, and 4 miRNAs were downregulated in both. Thus, the candidate markers from the DEmiRNA analysis totaled 24, comprising 12 upregulated and 12 downregulated miRNAs.