Featured Products
Our Promise to You
Guaranteed product quality, expert customer support
Immortalized Human Osteoprecursor Cells (OPC1)
- Specification
- Recommended Products
Cat.No.
CSC-I9257L
Description
The Immortalized Human Osteoprecursor Cells (OPC1) is a conditionally immortalized fetal osetoprecusor cell line uniquely expressing SV40 Tag in the presence of human interferon A/D which affects the proliferation capability. However, the Tag may be expressed constitutively in the presence of serum. It expresses alkaline phosphatase (ALP), osetocalcin (OC), osteonectin (ON), osteopontin (OP), procollagen-I (ProI), and PTH receptor (PTHR). The cells are able to differentiate in the presence of recombinant human BMP-2 (rhBMP2), a regulatory factor involved in differentiation. This cell line is valuable tool as a model system in studies in bone-graft substitutes.
Species
Homo sapiens
Source
Bone
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
Serial passaging and calcium phosphate-mediated transfection with a plasmid carrying SV40 T antigen, activated by human A/D interferon
Markers
Alkaline phosphatase (ALP), osetocalcin (OC), osteonectin (ON), osteopontin (OP), procollagen-I (ProI), and PTH receptor (PTHR)
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CIK-HT013 HT® Lenti-hTERT Immortalization Kit
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
1) RT-PCR analysis for osteoblast markers;
2) ALP staining was utilized to determine presence of ALP.
2) ALP staining was utilized to determine presence of ALP.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
- Q & A
- Customer Review
Customer Support & Price Inquiry
