Immortalized Human Osteoprecursor Cells (OPC1)

Cat.No.: CSC-I9257L

Species: Homo sapiens

Source: Bone

Morphology: Polygonal

Culture Properties: Adherent

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Cat.No.

CSC-I9257L

Description

The Immortalized Human Osteoprecursor Cells (OPC1) is a conditionally immortalized fetal osetoprecusor cell line uniquely expressing SV40 Tag in the presence of human interferon A/D which affects the proliferation capability. However, the Tag may be expressed constitutively in the presence of serum. It expresses alkaline phosphatase (ALP), osetocalcin (OC), osteonectin (ON), osteopontin (OP), procollagen-I (ProI), and PTH receptor (PTHR). The cells are able to differentiate in the presence of recombinant human BMP-2 (rhBMP2), a regulatory factor involved in differentiation. This cell line is valuable tool as a model system in studies in bone-graft substitutes.

Species

Homo sapiens

Source

Bone

Culture Properties

Adherent

Morphology

Polygonal

Immortalization Method

Serial passaging and calcium phosphate-mediated transfection with a plasmid carrying SV40 T antigen, activated by human A/D interferon

Markers

Alkaline phosphatase (ALP), osetocalcin (OC), osteonectin (ON), osteopontin (OP), procollagen-I (ProI), and PTH receptor (PTHR)

Applications

For Research Use Only

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Note: Never can cells be kept at -20 °C.

Shipping

Dry Ice.

Recommended Products

CIK-HT013 HT® Lenti-hTERT Immortalization Kit
CIK-HT003 HT® Lenti-SV40T Immortalization Kit

Quality Control

1) RT-PCR analysis for osteoblast markers;
2) ALP staining was utilized to determine presence of ALP.

BioSafety Level

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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