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Immortalized Human Nucleus Pulposus Non-Responder Cells
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Cat.No.
CSC-I9318L
Description
Immortalized Human Nucleus Pulposus Non-Responder Cells were derived from healthy disc materials from patients and were immortalized via retroviral transduction carrying the SV40LTAg and hTERT genes. The cells retained its primary phenotype and express the SOX9, COL2A1 and COL1A1 markers. It does not have the ability to undergo chondrogenic differentiation, unlike the Immortalized Human Nucleus Pulposus Responder Cells. The Immortalized Human Nucleus Pulposus Non-Responder Cells may be used to study the biology of the nucleus pulposus.
Species
Homo sapiens
Source
Disc Tissue
Culture Properties
Adherent
Morphology
Cobblestone, wave-like
Immortalization Method
Transduction with retroviral particles expressing SV40LTAg (pBABE-hygro) and hTERT (pBMN-IRES-NEO)
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Quality Control
1) 200 μg/ml G418 and 12 μg/ml hygromycin B for selection was used;
2) PCR utilized to detect chondrocyte markers;
3) Immortalization genes detected via immunoblot
2) PCR utilized to detect chondrocyte markers;
3) Immortalization genes detected via immunoblot
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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