Immortalized Human Gingival Fibroblasts-SV40

Immortalized Human Gingival Fibroblasts-SV40 are human gingival connective tissue cells immortalized by introduction of the SV40 Large T antigen, which confers extended proliferative capacity while retaining important biological characteristics of primary gingival fibroblasts. These cells have a typical spindle structure of fibroblasts and retain the ability to produce components of the extracellular matrix (ECM), respond to inflammatory stimuli, and participate in tissue remodeling processes.

Gingival fibroblasts are one of the principal cell types of gingival connective tissue and play a key role in the maintenance of homeostasis of the periodontal tissue, in the regulation of wound healing and in mediating host responses to microbial infection. The SV40 immortalization process allows to bypass the short life span of primordial cells and provides a stable and repeatable in vitro model for long-term investigations and high-throughput studies.

Immortalized Human Gingival Fibroblasts-SV40 are commonly employed in periodontal disease research, oral inflammatory studies, biomaterial evaluation and regenerative dentistry. They are useful instruments for studying host-pathogen interactions with oral bacteria, cytokine signaling pathways, extracellular matrix remodeling, and fibrosis-related mechanisms. These cells are also widely used to evaluate the biocompatibility of dental materials, implant surface changes and tissue engineering scaffolds.