 
            Immortalized Human Gingival Fibroblasts-SV40
Cat.No.: CSC-I2120Z
Species: homo sapiens
Morphology: Polygonal
Culture Properties: Adherent
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Cat.No.
CSC-I2120Z
Description
Immortalized Human Gingival Fibroblasts-SV40 have been obtained immortalizing Human Gingival Fibroblasts with Lenti-SV40 Lentivirus. Immortalized cells were controlled passaging side by side with the primary cells. Primary cells go into senescence after the 5th passage while the SV40 tranduced cells go beyond 30 passges.
Species
                                homo sapiens
                            Recommended Medium
                                SuperCult® Immortalized Human Gingival Fibroblast Medium (Cat No.: CM-I2120Z)
                            Freezing Medium
                                Complete medium supplemented with 10% (v/v) DMSO
                            Culture Properties
                                Adherent
                            Morphology
                                Polygonal
                            Immortalization Method
                                SV40 large T antigen
                            Growth Properties
                                Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
                            Shipping
                                Dry Ice.
                            Quality Control
                                Real Time PCR was used to quantify SV40T gene expression in immortalized cell line.
free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations
                            free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations
Storage and Shipping
                                Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20°C.
                            Note: Never can cells be kept at -20°C.
Citation Guidance
                        
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