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Immortalized Human Foreskin Keratinocytes (16E6/E7 HFK)
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Cat.No.
CSC-I9336L
Description
The Immortalized Human Foreskin Keratinocytes (16E6/E7 HFK) were derived from foreskin tissue of newborns and were transfected with a retrovirus expressing HPV-16 E7 and HPV-16 E6. The HPV-16 E6 and E7 delay cellular differentiation and keep the immortalized cells in a state of DNA synthesis even after they have entered the suprabasal layer of the epidermis. Serum-induced and calcium-induced differentiation of the Immortalized Human Foreskin Keratinocytes is also inhibited by HPV-16 E6. In these immortalized cells, the regulated genes by the HPV-16 E6 and E7 may play a role in the HPV life cycle and in HPV-mediated carcinogenesis which make the Immortalized Human Foreskin Keratinocytes (16E6/E7 HFK) valuable not only as a model in immunology but also in gene regulation studies.
Species
Homo sapiens
Source
Foreskin tissue
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
Transfection with retrovirus expressing HPV-16 E7 and HPV-16 E6
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
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Quality Control
1) ELISA to measure telomerase activity;
2) Microarray analysis of gene regulation
2) Microarray analysis of gene regulation
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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