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Immortalized Human Adrenocortical Cells-hTERT
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Cat.No.
CSC-I9190L
Description
The human adrenal cortex plays an important role in regulating the endocrine system by secreting glucocorticoids, mineralcorticoids and adrenal androgens in response to environmental stimulation. The Immortalized Human Adrenocorticol Cells- hTERT is established from primary cells (isolated from a healthy kidney donor) using retrovirus carrying hTERT gene. These cells are useful in studies involving the biochemical and molecular mechanisms associated with adrenal steroidogenesis and adrenal physiology.
Species
Homo sapiens
Source
Adrenal gland
Culture Properties
Adherent
Immortalization Method
Serial passaging and transduction with recombinant lentiviruses carrying hTERT gene
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
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CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
1. Telomerase repeat amplification protocol (TRAP) assay was performed to measure telomerase activity in the immortalized cell line. 2) RT-PCR was used to quantify telomerase RNA component (TERC) expression in the immortalized cell line.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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