AC-1M59

CSC-C0484

Subclone of ACH1P; ACH1P was established by somatic hybridization of primary male trophoblast cells and the HGPRT-defective choriocarcinoma cell line AC1-1 which was originally derived from cell line JEG-3; in contrast to the respective sister cell lines, AC-1M59 was described as expressing ERK5, cytokeratin I, TNF-R2, VEGFR-2 and IL-2

Human

choriocarcinoma-trophoblast hybrid

Ham's F-12 and 10% h.i. FBS.

monolayer

adherent epithelial-like cells growing as monolayer and carrying many intracellular dark granula

Human flat-moded hypertetraploid karyotype with 8% polyploidy - 86-97<4n>XXXY, -4, +7, -8, +9, -13, -13, -18, +19, +2-5mar, del(X)(p11p22), der(X)(Xpter->Xq2?8::4p1?->4p1?::11q1?1->11qter), der(7)t(7;7)(p22;q11.22)add(7)(q32), add(7)(q32), add(9)(p24), ad

Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, desmin -, endothel -, GFAP -, neurofilament -, vimentin +
Viruses: PCR: EBV -, HBV -,

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

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