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ACH1P
- Specification
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Cat.No.
CSC-C0496
Description
Established by somatic hybridization of primary male trophoblast cells and the HGPRT-defective choriocarcinoma cell line AC1-1 which was originally derived from cell line JEG-3
Species
Human
Source
choriocarcinoma-trophoblast hybrid
Recommended Medium
Ham's F-12 and 10% h.i. FBS.
Culture Properties
monolayer
Morphology
adherent epithelial-like cells growing as monolayers, cells carry a large number of dark granula
Karyotype
Human flat-moded hypertetraploid karyotype with 10% polyploidy - 85-104<4n>XXY/XXYY/XXXY, +2, +5, +7, -10, -11, +12, -13, +16, -18, +21, +1-4 mar, add(7)(q32), i(13q), add(15)(p11) add(19)(p13)x1-2 - sideline with del(X)(p11p22), der(X)(Xpter->Xq2?8::4p1?
Quality Control
Mycoplasma: contamination was eliminated with ciprofloxacin, then negative in microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, desmin -, endothel -, GFAP -, neurofilament -, vimentin -
Viruses: PCR: EBV -, HBV -, HCV -, HI
Immunology: cytokeratin +, desmin -, endothel -, GFAP -, neurofilament -, vimentin -
Viruses: PCR: EBV -, HBV -, HCV -, HI
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
- Q & A
- Customer Review
Q:
What are the considerations for using EMEM in different cell culture applications?
A:
Considerations include the specific nutritional requirements of the cell type, the addition of serum or other supplements, and the need to maintain stable pH and osmolality.
Average Rating: 5.0 | 1 Scientist has reviewed this product
Trusted results
The quality and consistency of these cell products have greatly improved the reliability of our cellular applications, ensuring the generation of trustworthy and meaningful results.
12 Oct 2023
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