ACH1P

Cat.No.: CSC-C0496

Species: Homo sapiens (Human)

Morphology: adherent epithelial-like cells growing as monolayers, cells carry a large number of dark granula

Culture Properties: monolayer

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Cat.No.

CSC-C0496

Description

Established by somatic hybridization of primary male trophoblast cells and the HGPRT-defective choriocarcinoma cell line AC1-1 which was originally derived from cell line JEG-3

Species

Homo sapiens (Human)

Recommended Medium

Ham's F-12 and 10% h.i. FBS.

Culture Properties

monolayer

Morphology

adherent epithelial-like cells growing as monolayers, cells carry a large number of dark granula

Karyotype

Human flat-moded hypertetraploid karyotype with 10% polyploidy - 85-104<4n>XXY/XXYY/XXXY, +2, +5, +7, -10, -11, +12, -13, +16, -18, +21, +1-4 mar, add(7)(q32), i(13q), add(15)(p11) add(19)(p13)x1-2 - sideline with del(X)(p11p22), der(X)(Xpter->Xq2?8::4p1?

Quality Control

Mycoplasma: contamination was eliminated with ciprofloxacin, then negative in microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, desmin -, endothel -, GFAP -, neurofilament -, vimentin -
Viruses: PCR: EBV -, HBV -, HCV -, HI

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Synonyms

ACH-1P

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.