Description
Established by somatic hybridization of primary male trophoblast cells and the HGPRT-defective choriocarcinoma cell line AC1-1 which was originally derived from cell line JEG-3
Karyotype
Human flat-moded hypertetraploid karyotype with 10% polyploidy - 85-104<4n>XXY/XXYY/XXXY, +2, +5, +7, -10, -11, +12, -13, +16, -18, +21, +1-4 mar, add(7)(q32), i(13q), add(15)(p11) add(19)(p13)x1-2 - sideline with del(X)(p11p22), der(X)(Xpter->Xq2?8::4p1?
Source
choriocarcinoma-trophoblast hybrid
Culture Properties
monolayer
Morphology
adherent epithelial-like cells growing as monolayers, cells carry a large number of dark granula
Quality Control
Mycoplasma: contamination was eliminated with ciprofloxacin, then negative in microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, desmin -, endothel -, GFAP -, neurofilament -, vimentin -
Viruses: PCR: EBV -, HBV -, HCV -, HI
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
USA: 1-631-626-9181
Europe: 44-207-097-1828
