AC-1M88

Cat.No.: CSC-C0486

Species: Homo sapiens (Human)

Morphology: epitheloid cells growing adherently in monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C0486

Description

One of five phenotypically and cytogenetically distinct subclones of a hybrid (ACH1P) formed by fusing an HGPRT-defective mutant (AC1-1) of JEG-3 human choriocarcinoma with primary human trophoblast (chorion laeve) cells

Species

Homo sapiens (Human)

Recommended Medium

Ham's F-12 and 10% h.i. FBS.

Culture Properties

monolayer

Morphology

epitheloid cells growing adherently in monolayers

Karyotype

Human flat-moded hypertetraploid karyotype with 11% polyploidy - 80-99<4n>XXX, -Y, -3, +7, +7, -8, +9, +9, +12, -13, +3-5mar, del(X)(p11p22), der(7)t(7;7)(p22;q11.22)add(7)(q32)x2, add(7)(q32), add(9)(p24), i(13q), add(19)(p13), der(19)t(11;19)(q11;q13) -

Quality Control

Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, desmin -, endothel -, GFAP -, neurofilament -, vimentin +
Viruses: PCR: EBV -, HBV -,

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.